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小鼠白介素6酶免試劑盒,(IL-6)ELISA檢測試劑盒,英文名:IL-6 ELISA Kit,英文縮寫:IL-6 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素6ELISA檢測試劑盒,IL-6 檢測試劑盒,IL-6 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素6酶免試劑盒,(IL-6)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
鈣蛋白酶1(CAPN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpain 1, Large Subunit (CAPN1)48T/96TY11135Mus musculus (Mouse)
花生四烯酸-5-脂加氧酶(ALOX5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)48T/96TY11136Rattus norvegicus (Rat)
ⅡD組磷脂酶A2(PLA2G2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Group IID (PLA2G2D)48T/96TY11137Mus musculus (Mouse)
胞漿型磷脂酶A2(cPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Cytosolic (cPLA2)48T/96TY11138Mus musculus (Mouse)
血幼素(HJV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hemojuvelin (HJV)48T/96TY11139Homo sapiens (Human)
轉(zhuǎn)甲狀腺素蛋白(TTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transthyretin (TTR)48T/96TY11140Rattus norvegicus (Rat)
白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Albumin (ALB)48T/96TY11141Rattus norvegicus (Rat)
組蛋白H3(H3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histone H3 (H3)48T/96TY11142Mus musculus (Mouse)
多配體蛋白聚糖1(SDC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Syndecan 1 (SDC1)48T/96TY11143Homo sapiens (Human)
載脂蛋白A4(APOA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A4 (APOA4)48T/96TY11144Sus scrofa; Porcine (Pig)
載脂蛋白D(APOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein D (APOD)48T/96TY11145Homo sapiens (Human)
補體成分5(C5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5 (C5)48T/96TY11146Homo sapiens (Human)
血小板反應(yīng)蛋白解整合素金屬肽酶1(ADAMTS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)48T/96TY11147Homo sapiens (Human)
補體成分8γ(C8γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 8g (C8g)48T/96TY11148Homo sapiens (Human)
血小板反應(yīng)蛋白解整合素金屬肽酶7(ADAMTS7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)48T/96TY11149Homo sapiens (Human)
蛋白S(PROS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein S (PROS)48T/96TY11150Homo sapiens (Human)
色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pigment Epithelium Derived Factor (PEDF)48T/96TY11151Homo sapiens (Human)
表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein B (SPB)48T/96TY11152Bos taurus; Bovine (Cattle)
表面活性物質(zhì)關(guān)聯(lián)蛋白C(SPC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein C (SPC)48T/96TY11153Bos taurus; Bovine (Cattle)
瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY11154Chicken (Gallus)
基質(zhì)金屬蛋白酶28(MMP28)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 28 (MMP28)48T/96TY11155Homo sapiens (Human)
肌腱蛋白C(TNC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tenascin C (TNC)48T/96TY11156Homo sapiens (Human)
肌腱蛋白C(TNC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tenascin C (TNC)48T/96TY11157Rattus norvegicus (Rat)
凝血因子ⅩⅢB肽(F13B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XIII B Polypeptide (F13B)48T/96TY11158Homo sapiens (Human)
膜聯(lián)蛋白A2(ANXA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A2 (ANXA2)48T/96TY11159Mus musculus (Mouse)
膜聯(lián)蛋白A2(ANXA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A2 (ANXA2)48T/96TY11160Rattus norvegicus (Rat)
羧甲基賴氨酸(CML)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carboxymethyl Lysine (CML)48T/96TY11161General
鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepcidin (Hepc)48T/96TY11162Homo sapiens (Human)
白介素33(IL33)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 33 (IL33)48T/96TY11163Homo sapiens (Human)
內(nèi)皮素受體A(ETRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelin Receptor A (ETRA)48T/96TY11164Rattus norvegicus (Rat)
內(nèi)皮素受體B(ETRB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelin Receptor B (ETRB)48T/96TY11165Rattus norvegicus (Rat)
微管關(guān)聯(lián)蛋白τ(MAPτ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein Tau (MAPt)48T/96TY11166Homo sapiens (Human)
τ-蛋白激酶1(τPK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tau-Protein Kinase 1 (tPK1)48T/96TY11167Homo sapiens (Human)
強啡肽(Dyn)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Big Dynorphin (Dyn)48T/96TY11168Rattus norvegicus (Rat)
甘露糖結(jié)合蛋白(MBL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Binding Lectin (MBL)48T/96TY11169Mus musculus (Mouse)
甘露聚糖結(jié)合凝集素關(guān)聯(lián)絲氨酸蛋白酶1(MASP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Associated Serine Protease 1 (MASP1)48T/96TY11170Mus musculus (Mouse)
甘露聚糖結(jié)合凝集素關(guān)聯(lián)絲氨酸蛋白酶1(MASP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Associated Serine Protease 1 (MASP1)48T/96TY11171Rattus norvegicus (Rat)
上皮型脂肪酸結(jié)合蛋白(FABP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 5, Epidermal (FABP5)48T/96TY11172Homo sapiens (Human)
凋亡相關(guān)因子配體(FASL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis Ligand (FASL)48T/96TY11173Sus scrofa; Porcine (Pig)
血紅素結(jié)合蛋白(HPX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hemopexin (HPX)48T/96TY11174Homo sapiens (Human)
蛋白激酶N2(PKN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase N2 (PKN2)48T/96TY11175Homo sapiens (Human)
蛋白激酶N2(PKN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase N2 (PKN2)48T/96TY11176Rattus norvegicus (Rat)
蛋白激酶N2(PKN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase N2 (PKN2)48T/96TY11177Mus musculus (Mouse)
CD3d分子(CD3d)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 3d (CD3d)48T/96TY11178Homo sapiens (Human)
Toll樣受體1(TLR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 1 (TLR1)48T/96TY11179Homo sapiens (Human)
Toll樣受體3(TLR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 3 (TLR3)48T/96TY11180Homo sapiens (Human)
骨形成蛋白1(BMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 1 (BMP1)48T/96TY11181Bos taurus; Bovine (Cattle)
Toll樣受體5(TLR5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 5 (TLR5)48T/96TY11182Homo sapiens (Human)
Toll樣受體8(TLR8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 8 (TLR8)48T/96TY11183Homo sapiens (Human)
Toll樣受體10(TLR10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 10 (TLR10)48T/96TY11184Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。