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小鼠小扁豆素結(jié)合型甲胎蛋白/甲胎蛋白異質(zhì)體2酶免試劑盒,(AFP-L2)ELISA檢測試劑盒 ,英文名:AFP-L2 ELISA Kit ,英文縮寫:AFP-L2 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠小扁豆素結(jié)合型甲胎蛋白/甲胎蛋白異質(zhì)體2ELISA檢測試劑盒,AFP-L2 檢測試劑盒, AFP-L2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠小扁豆素結(jié)合型甲胎蛋白/甲胎蛋白異質(zhì)體2酶免試劑盒,(AFP-L2)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
激活蛋白C(APC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activated Protein C (APC)48T/96TY12085Mus musculus (Mouse)
促卵泡素(FSH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follicle Stimulating Hormone (FSH)48T/96TY12086Rattus norvegicus (Rat)
載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein E (APOE)48T/96TY12087Sus scrofa; Porcine (Pig)
氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)48T/96TY12088Rattus norvegicus (Rat)
氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)48T/96TY12089Canis familiaris; Canine (Dog)
CD24分子(CD24)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 24 (CD24)48T/96TY12090Homo sapiens (Human)
生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone (GH)48T/96TY12091Bos taurus; Bovine (Cattle)
白介素19(IL19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 19 (IL19)48T/96TY12092Mus musculus (Mouse)
透明質(zhì)酸酶(HAase)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hyaluronidase (HAase)48T/96TY12093Homo sapiens (Human)
白介素22(IL22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 22 (IL22)48T/96TY12094Mus musculus (Mouse)
白細胞介素24(IL24)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 24 (IL24)48T/96TY12095Mus musculus (Mouse)
角化粒(CDSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corneodesmosin (CDSN)48T/96TY12096Homo sapiens (Human)
抑制素A(INHA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin A (INHA)48T/96TY12097Sus scrofa; Porcine (Pig)
抑制素B(INHB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin B (INHB)48T/96TY12098Sus scrofa; Porcine (Pig)
早期生長應(yīng)答因子2(EGR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early Growth Response Protein 2 (EGR2)48T/96TY12099Mus musculus (Mouse)
唾液酸結(jié)合Ig樣凝集素1(SIGLEC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Binding Ig Like Lectin 1 (SIGLEC1)48T/96TY12100Homo sapiens (Human)
降鈣素(CT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcitonin (CT)48T/96TY12101Canis familiaris; Canine (Dog)
堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY12102Bos taurus; Bovine (Cattle)
生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone (GH)48T/96TY12103Equus caballus; Equine (Horse)
基質(zhì)金屬蛋白酶12(MMP12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 12 (MMP12)48T/96TY12104Mus musculus (Mouse)
堿性磷酸酶(ALP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY12105Oryctolagus cuniculus (Rabbit)
血管內(nèi)皮生長因子受體3(VEGFR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 3 (VEGFR3)48T/96TY12106Bos taurus; Bovine (Cattle)
表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Surfactant Associated Protein D (SPD)48T/96TY12107Mus musculus (Mouse)
紅細胞補體受體1(CR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Receptor 1, Erythrocyte (CR1)48T/96TY12108Homo sapiens (Human)
細胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(EMMPRIN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Extracellular Matrix Metalloproteinase Inducer (EMMPRIN)48T/96TY12109Homo sapiens (Human)
胃動素(MTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Motilin (MTL)48T/96TY12110Bos taurus; Bovine (Cattle)
纖維蛋白原(FG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen (FG)48T/96TY12111Sus scrofa; Porcine (Pig)
肝細胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY12112Canis familiaris; Canine (Dog)
脂聯(lián)素受體2(ADIPOR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 2 (ADIPOR2)48T/96TY12113Homo sapiens (Human)
氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP)48T/96TY12114Oryctolagus cuniculus (Rabbit)
脂聯(lián)素(ADP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin (ADP)48T/96TY12115Canis familiaris; Canine (Dog)
組蛋白脫乙?;?(HDAC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histone Deacetylase 2 (HDAC2)48T/96TY12116Homo sapiens (Human)
血管緊張素Ⅲ(AngⅢ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin III (AngIII)48T/96TY12117Homo sapiens (Human)
脊髓灰質(zhì)炎病毒受體相關(guān)分子4(PVRL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poliovirus Receptor Related Protein 4 (PVRL4)48T/96TY12118Homo sapiens (Human)
胞漿磷蛋白1(STMN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stathmin 1 (STMN1)48T/96TY12119Homo sapiens (Human)
松弛肽(RLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Relaxin (RLN)48T/96TY12120Bos taurus; Bovine (Cattle)
抗乙酸膽堿受體抗體(Anti-AChR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Acetylcholine Receptor Antibody (Anti-AChR)48T/96TY12121Homo sapiens (Human)
Slit同源物1(Slit1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 1 (Slit1)48T/96TY12122Homo sapiens (Human)
Ⅰ型前膠原(PCⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen I (PCI)48T/96TY12123Homo sapiens (Human)
Ⅲ型前膠原(PCⅢ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen III (PCIII)48T/96TY12124Homo sapiens (Human)
攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nesfatin 1 (NES1)48T/96TY12125Rattus norvegicus (Rat)
α白蛋白(AFM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Afamin (AFM)48T/96TY12126Mus musculus (Mouse)
催產(chǎn)素受體(OXTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxytocin Receptor (OXTR)48T/96TY12127Homo sapiens (Human)
熱休克轉(zhuǎn)錄因子2(HSF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Transcription Factor 2 (HSF2)48T/96TY12128Homo sapiens (Human)
β2-微球蛋白(β2M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-2-Microglobulin (b2M)48T/96TY12129Mus musculus (Mouse)
谷胱甘肽S轉(zhuǎn)移酶ω1(GSTω1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Omega 1 (GSTo1)48T/96TY12130Homo sapiens (Human)
C-型凝集素域家族4成員K(CLEC4K)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C-Type Lectin Domain Family 4, Member K (CLEC4K)48T/96TY12131Homo sapiens (Human)
N-酰基鞘氨醇酰胺水解酶1(ASAH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acylsphingosine Amidohydrolase 1 (ASAH1)48T/96T,(AFP-L2)ELISA檢測試劑盒Y12132Homo sapiens (Human)
兜甲蛋白(LOR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Loricrin (LOR)48T/96TY12133Homo sapiens (Human)
鞘脂激活蛋白原(PSAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prosaposin (PSAP)48T/96TY12134Homo sapiens (Human)
各種類型ELISA測定時,,(AFP-L2)ELISA檢測試劑盒一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。