服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠高鐵血紅蛋白酶免試劑盒,(MHB)ELISA檢測試劑盒 ,英文名:MHB ELISA Kit ,英文縮寫:MHB ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠高鐵血紅蛋白ELISA檢測試劑盒,MHB 檢測試劑盒, MHB 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠高鐵血紅蛋白酶免試劑盒,(MHB)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
補體成分9(C9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 9 (C9)48T/96TY12535Homo sapiens (Human)
硬骨素(SOST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sclerostin (SOST)48T/96TY12536Mus musculus (Mouse)
葡萄糖6磷酸異構(gòu)酶(GPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose 6 Phosphate Isomerase (GPI)48T/96TY12537Mus musculus (Mouse)
鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepcidin (Hepc)48T/96TY12538Bos taurus; Bovine (Cattle)
頭蛋白(NOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Noggin (NOG)48T/96TY12539Mus musculus (Mouse)
神經(jīng)肽FF(NPFF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropeptide FF (NPFF)48T/96TY12540Mus musculus (Mouse)
腎連蛋白(NPNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nephronectin (NPNT)48T/96TY12541Homo sapiens (Human)
白介素1受體輔助蛋白(IL1RAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP)48T/96TY12542Homo sapiens (Human)
髓細胞觸發(fā)受體2(TREM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)48T/96TY12543Homo sapiens (Human)
泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)48T/96TY12544Homo sapiens (Human)
HtrA絲氨酸肽酶1(HTRA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for HtrA Serine Peptidase 1 (HTRA1)48T/96TY12545Homo sapiens (Human)
肉毒堿乙酰轉(zhuǎn)移酶(CRAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Acetyltransferase (CRAT)48T/96TY12546Mus musculus (Mouse)
絲氨酸肽酶抑制因子Kazal型1(SPINK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serine Peptidase Inhibitor Kazal Type 1 (SPINK1)48T/96TY12547Homo sapiens (Human)
鈣蛋白酶抑制蛋白(CAST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpastatin (CAST)48T/96TY12548Mus musculus (Mouse)
脯氨酰4-羥化酶α多肽Ⅲ(P4Hα3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3)48T/96TY12549Homo sapiens (Human)
干擾素γ誘導(dǎo)蛋白30(IFI30)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma Inducible Protein 30 (IFI30)48T/96TY12550Homo sapiens (Human)
ATP結(jié)合盒轉(zhuǎn)運蛋白A1(ABCA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter A1 (ABCA1)48T/96TY12551Mus musculus (Mouse)
碳酸酐酶Ⅸ(CA9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase IX (CA9)48T/96TY12552Mus musculus (Mouse)
味覺受體1型成員2(TAS1R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Taste Receptor Type 1 Member 2 (TAS1R2)48T/96TY12553Mus musculus (Mouse)
輕肽神經(jīng)絲蛋白(NEFL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurofilament, Light Polypeptide (NEFL)48T/96TY12554Homo sapiens (Human)
凝血因子Ⅻ(F12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XII (F12)48T/96TY12555Mus musculus (Mouse)
谷胱甘肽S轉(zhuǎn)移酶α3(GSTα3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 3 (GSTa3)48T/96TY12556Rattus norvegicus (Rat)
IgE-Fc片段受體Ⅱ(FcεRⅡ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin E (FceRII)48T/96TY12557Mus musculus (Mouse)
色胺酸羥化酶1(TPH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tryptophan Hydroxylase 1 (TPH1)48T/96TY12558Rattus norvegicus (Rat)
視黃醇結(jié)合蛋白1(RBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinol Binding Protein 1, Cellular (RBP1)48T/96TY12559Homo sapiens (Human)
G蛋白β1(GNβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for G Protein Beta 1 (GNb1)48T/96TY12560Mus musculus (Mouse)
轉(zhuǎn)化生長因子β受體Ⅱ(TGFβR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Receptor II (TGFbR2)48T/96TY12561Homo sapiens (Human)
死骨片1(SQSTM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sequestosome 1 (SQSTM1)48T/96TY12562Homo sapiens (Human)
胃內(nèi)因子(GIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastric Intrinsic Factor (GIF)48T/96TY12563Homo sapiens (Human)
前蛋白轉(zhuǎn)化酶枯草溶菌素1(PCSK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1)48T/96TY12564Homo sapiens (Human)
細胞維甲酸結(jié)合蛋白2(CRABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cellular Retinoic Acid Binding Protein 2 (CRABP2)48T/96TY12565Homo sapiens (Human)
驅(qū)動蛋白家族成員1A(KIF1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kinesin Family, Member 1A (KIF1A)48T/96TY12566Homo sapiens (Human)
頭蛋白(NOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Noggin (NOG)48T/96TY12567Rattus norvegicus (Rat)
載脂蛋白B100(APOB100)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein B100 (APOB100)48T/96TY12568Bos taurus; Bovine (Cattle)
白介素25(IL25)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 25 (IL25)48T/96TY12569Mus musculus (Mouse)
胱天蛋白酶1(CASP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 1 (CASP1)48T/96TY12570Rattus norvegicus (Rat)
成纖維細胞生長因子15(FGF15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 15 (FGF15)48T/96TY12571Mus musculus (Mouse)
線粒體解偶聯(lián)蛋白1(UCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)48T/96TY12572Mus musculus (Mouse)
分泌型免疫球蛋白A(sIgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretory Immunoglobulin A (sIgA)48T/96TY12573Bos taurus; Bovine (Cattle)
趨化因子C-X-C-基元受體4(CXCR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Receptor 4 (CXCR4)48T/96TY12574Sus scrofa; Porcine (Pig)
信號素3A(SEMA3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Semaphorin 3A (SEMA3A)48T/96TY12575Homo sapiens (Human)
神經(jīng)束蛋白(NFASC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurofascin (NFASC)48T/96TY12576Homo sapiens (Human)
肉毒堿棕櫚?;D(zhuǎn)移酶2(CPT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2)48T/96TY12577Homo sapiens (Human)
膜聯(lián)蛋白A3(ANXA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A3 (ANXA3)48T/96TY12578Homo sapiens (Human)
肉毒堿棕櫚酰基轉(zhuǎn)移酶1B(CPT1B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 1B, Muscle (CPT1B)48T/96TY12579Homo sapiens (Human)
四旋蛋白1(TSPAN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tetraspanin 1 (TSPAN1)48T/96TY12580Homo sapiens (Human)
亮氨酸豐富重復(fù)LGI家族成員3(LGI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucine Rich Repeat LGI Family, Member 3 (LGI3)48T/96TY12581Mus musculus (Mouse)
白介素1受體輔助蛋白樣蛋白2(IL1RAPL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2)48T/96TY12582Homo sapiens (Human)
分泌性白細胞蛋白酶抑制因子(SLPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)48T/96TY12583Mus musculus (Mouse)
天冬酰胺內(nèi)肽酶(LGMN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Legumain (LGMN)48T/96TY12584Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。