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小鼠氧化型谷胱甘肽酶免試劑盒,(GSGS)ELISA檢測試劑盒 ,英文名:GSGS ELISA Kit ,英文縮寫:GSGS ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠氧化型谷胱甘肽ELISA檢測試劑盒,GSGS 檢測試劑盒, GSGS 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠氧化型谷胱甘肽酶免試劑盒,(GSGS)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
腸堿性磷酸酶(ALPI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Intestinal (ALPI)48T/96TY12835Rattus norvegicus (Rat)
成纖維細胞生長因子11(FGF11)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 11 (FGF11)48T/96TY12836Homo sapiens (Human)
成纖維細胞生長因子12(FGF12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 12 (FGF12)48T/96TY12837Homo sapiens (Human)
VGF神經生長因子誘導蛋白(VGF)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for VGF Nerve Growth Factor Inducible (VGF)48T/96TY12838Mus musculus (Mouse)
熱休克蛋白β8(HSPβ8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)48T/96TY12839Rattus norvegicus (Rat)
Nexn結合蛋白(NEXN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nexilin (NEXN)48T/96TY12840Homo sapiens (Human)
激肽原1(KNG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kininogen 1 (KNG1)48T/96TY12841Mus musculus (Mouse)
跨膜蛋白27(TMEM27)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transmembrane Protein 27 (TMEM27)48T/96TY12842Mus musculus (Mouse)
跨膜蛋白27(TMEM27)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transmembrane Protein 27 (TMEM27)48T/96TY12843Rattus norvegicus (Rat)
胸腎表達趨化因子(BRAK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK)48T/96TY12844Mus musculus (Mouse)
11-β-羥基類固醇脫氫酶1(HSD11β1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)48T/96TY12845Homo sapiens (Human)
G補綴FHA域血管生成因子1(AGGF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1)48T/96TY12846Homo sapiens (Human)
成纖維細胞生長因子13(FGF13)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 13 (FGF13)48T/96TY12847Homo sapiens (Human)
亮氨酰氨基肽酶(LAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leucine Aminopeptidase (LAP)48T/96TY12848Rattus norvegicus (Rat)
脂肪細胞型脂肪酸結合蛋白(FABP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)48T/96TY12849Rattus norvegicus (Rat)
谷氨酰胺酶(GLS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaminase (GLS)48T/96TY12850Mus musculus (Mouse)
谷氨酰胺酶2(GLS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaminase 2 (GLS2)48T/96TY12851Mus musculus (Mouse)
肌醇加氧酶(MIOX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myo Inositol Oxygenase (MIOX)48T/96TY12852Homo sapiens (Human)
核纖層蛋白A/C(LMNA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lamin A/C (LMNA)48T/96TY12853Rattus norvegicus (Rat)
氨乙酰丙酸δ合酶1(ALAS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aminolevulinate Delta Synthase 1 (ALAS1)48T/96TY12854Rattus norvegicus (Rat)
α1-B-糖蛋白(α1BG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-1-B-Glycoprotein (a1BG)48T/96TY12855Homo sapiens (Human)
心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)48T/96TY12856Mus musculus (Mouse)
印度刺猬因子(IHH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Indian (IHH)48T/96TY12857Homo sapiens (Human)
衰老關鍵蛋白1(FBLN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibulin 1 (FBLN1)48T/96TY12858Homo sapiens (Human)
NK6同源框蛋白1(NKX6-1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for NK6 Homeobox Protein 1 (NKX6-1)48T/96TY12859Homo sapiens (Human)
橋粒芯糖蛋白2(DSG2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Desmoglein 2 (DSG2)48T/96TY12860Homo sapiens (Human)
精氨酸加壓素受體1B(AVPR1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arginine Vasopressin Receptor 1B (AVPR1B)48T/96TY12861Mus musculus (Mouse)
含卷曲螺旋域蛋白3(CCDC3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Coiled Coil Domain Containing Protein 3 (CCDC3)48T/96TY12862Homo sapiens (Human)
脂肪酸去飽和酶2(FADS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Desaturase 2 (FADS2)48T/96TY12863Homo sapiens (Human)
脂肪酸去飽和酶2(FADS2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Desaturase 2 (FADS2)48T/96TY12864Rattus norvegicus (Rat)
脂肪酸去飽和酶1(FADS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Desaturase 1 (FADS1)48T/96TY12865Homo sapiens (Human)
脂肪酸去飽和酶1(FADS1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Desaturase 1 (FADS1)48T/96TY12866Rattus norvegicus (Rat)
IgA-Fc斷片受體(FcaR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fc Fragment Of IgA Receptor (FcaR)48T/96TY12867Homo sapiens (Human)
谷氨酸脫羧酶樣蛋白1(GADL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Decarboxylase Like Protein 1 (GADL1)48T/96TY12868Homo sapiens (Human)
腎母細胞瘤蛋白1(WT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wilms Tumor Protein (WT1)48T/96TY12869Homo sapiens (Human)
脂肪酸去飽和酶3(FADS3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Desaturase 3 (FADS3)48T/96TY12870Homo sapiens (Human)
谷氨酰胺酰肽環(huán)轉移酶(QPCT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaminyl Peptide Cyclotransferase (QPCT)48T/96TY12871Homo sapiens (Human)
無翅型MMTV整合位點家族成員10A(WNT10A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10A (WNT10A)48T/96TY12872Homo sapiens (Human)
無翅型MMTV整合位點家族成員7B(WNT7B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)48T/96TY12873Homo sapiens (Human)
無翅型MMTV整合位點家族成員7A(WNT7A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 7A (WNT7A)48T/96TY12874Homo sapiens (Human)
無翅型MMTV整合位點家族成員10B(WNT10B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)48T/96TY12875Homo sapiens (Human)
整合素關聯蛋白(IAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Integrin Associated Protein (IAP)48T/96TY12876Homo sapiens (Human)
62kDa核孔蛋白(NUP62)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nucleoporin 62kDa (NUP62)48T/96TY12877Homo sapiens (Human)
核呼吸因子1(NRF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nuclear Respiratory Factor 1 (NRF1)48T/96TY12878Rattus norvegicus (Rat)
沙漠刺猬因子(DHH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Desert (DHH)48T/96TY12879Homo sapiens (Human)
生長停滯DNA損傷可誘導蛋白α(GADD45α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)48T/96TY12880Homo sapiens (Human)
生長停滯DNA損傷可誘導蛋白α(GADD45α)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)48T/96TY12881Rattus norvegicus (Rat)
內磺肽α(ENSα)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Endosulfine Alpha (ENSa)48T/96TY12882Mus musculus (Mouse)
絡絲蛋白(RL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Reelin (RL)48T/96TY12883Homo sapiens (Human)
激肽釋放酶12(KLK12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 12 (KLK12)48T/96TY12884Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。